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Image Search Results
Journal: Cell Death & Disease
Article Title: NLRP3 activation induces ASC-dependent programmed necrotic cell death, which leads to neutrophilic inflammation
doi: 10.1038/cddis.2013.169
Figure Lengend Snippet: Cell death induced by CAPS-associated NLRP3 mutants was programmed cell death. ( a and b ) WT and mutant NLRP3-Tet-On-MC/9 cells were treated with 1 μ g/ml doxycycline for 12 h in addition to cathepsin B inhibitor CA074-Me (20 μ M), pan-caspase inhibitor Z-VAD-fmk (10 μ M), caspase-1 inhibitor Z-YVAD-cho (10 μ M), caspase-2 inhibitor, Z-VDVAD-fmk (10 μ M), caspase-3/7 inhibitor Z-DEVD-fmk (10 μ M), caspase-4 inhibitor Z-LEVD-fmk (10 μ M), caspase-8 inhibitor Z-IETD-fmk (10 μ M), caspase-9/11 inhibitor Z-LEHD-fmk (10 μ M) or caspase-12 inhibitor Z-ATAD-fmk (10 μ M). Cells were stained with 100 nM TOTO-3 for 5 min and analyzed by flow cytometry. ( c ) WT and mutant NLRP3-Tet-on-MC/9 cells were treated with 1 μ g/ml doxycycline for 12 h in addition to pan-caspase inhibitor Z-VAD-fmk or Boc-D-fmk (10/20/40 μ M). **, P <0.01; and ****, P <0.0001
Article Snippet: The pan-caspase inhibitors Z-VAD-fmk (R&D Systems), ac-VAD-cho (MERCK, Whitehouse Station, NJ, USA), Q-VD-OPh and Boc-D-fmk (BioVision, Milpitas, CA, USA), and specific caspase inhibitors Z-YVAD-fmk (MERCK), Z-VDVAD-fmk, Z-LEVD-fmk and
Techniques: Mutagenesis, Staining, Flow Cytometry